Proteomic profiling of urinary small extracellular vesicles in children with pneumonia: a pilot study.

ABSTRACT:

Background

Small extracellular vesicles (sEV) play a crucial role in immune responses to viral infection. However, the composition of sEV derived from children with viral pneumonia remains ill defined.

Methods

First, we performed mass spectrometry-based label-free proteomic analysis of urinary sEV in 7 children with viral pneumonia, 4 children with Mycoplasma pneumoniae pneumonia and 20 healthy children. Then a total of 33 proteins were selected to validate by multiple reaction monitoring analysis in an independent cohort of 20 healthy children and 29 children with pneumonia.

Results

In the discovery phase, a total of 1621 proteins were identified, while 260 proteins have differential expression in children with viral pneumonia compared to healthy children. Biological

SUBMITTER: Cheng J 

PROVIDER: S-EPMC9838271 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature