A 1-min double embedding method for small tissue specimens preserves comedone histology and eliminates the need for punch biopsies.

ABSTRACT:

Background

It is difficult to preserve the structure and microbial distribution inside comedonal plugs during routine processing.

Objective

The objective of this study is to determine the optimal method to preserve the comedonal corneum plug structure and inherent microorganisms thereby eliminating the need to perform punch biopsies in relevant studies.

Methods

Corneum plugs were extracted from comedones of acne vulgaris patients. Primary embedding using either a 2% agarose, 2% agar, 25% gelatin, or 2% agar + 2.5% gelatin solution was subsequently performed and the results compared. The specimens were then fixed, waxed, sectioned, and examined by light, fluorescence, and scanning electron microscopies to observe the structures and microorganisms within the plugs.

R

SUBMITTER: Xu DT 

PROVIDER: S-EPMC9838743 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature