Novel cell culture system for monitoring cells during continuous and variable negative-pressure wound therapy.

ABSTRACT:

Background

Although the clinical efficacy of negative-pressure wound therapy (NPWT) is well known, many of its molecular biological mechanisms remain unresolved, mainly due to the difficulty and paucity of relevant in vitro studies. We attempted to develop an in vitro cell culture system capable of real-time monitoring of cells during NPWT treatment.

Materials and methods

A novel negative-pressure cell culture system was developed by combining an inverted microscope, a stage-top incubator, a sealed metal chamber for cell culture, and an NPWT treatment device. Human keratinocytes, PSVK-1, were divided into ambient pressure (AP), continuous negative-pressure (NPc), and intermittent negative-pressure (NPi) groups and cultured for 24 h with scratch assay using our real-time moni

SUBMITTER: Yamashiro T 

PROVIDER: S-EPMC9838773 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature