Diversity and functional analysis of rumen and fecal microbial communities associated with dietary changes in crossbreed dairy cattle.

ABSTRACT: The objective of this study was to investigate the effect of varying roughage and concentrate proportions, in diet of crossbreed dairy cattle, on the composition and associated functional genes of rumen and fecal microbiota. We also explored fecal samples as a proxy for rumen liquor samples. Six crossbred dairy cattle were reared on three diets with an increasing concentrate and reducing roughage amount in three consecutive 10-day periods. After each period, individual rumen liquor and fecal samples were collected and analyzed through shotgun metagenomic sequencing. Average relative abundance of identified Operational Taxonomic Units (OTU) and microbial functional roles from all animals were compared between diets and sample types (fecal and rumen liquor). Results indicated that dietary mo

SUBMITTER: Kibegwa FM 

PROVIDER: S-EPMC9838872 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature