A tamoxifen-inducible Cre knock-in mouse for lens-specific gene manipulation.

ABSTRACT: Mouse models are valuable tools in studying lens biology and biochemistry, and the Cre-loxP system is the most used technology for gene targeting in the lens. However, numerous genes are indispensable in lens development. The conventional knockout method either prevents lens formation or causes simultaneous cataract formation, hindering the studies of their roles in lens structure, growth, metabolism, and cataractogenesis during lens aging. An inducible Cre-loxP mouse line is an excellent way to achieve such a purpose. We established a lens-specific Cre ERT2 knock-in mouse (LCEK), an inducible mouse model for lens-specific gene targeting in a spatiotemporal manner. LCEK mice were created by in-frame infusion of a P2A-CreER^T2 at the C-terminus of the last coding exon of the gene

SUBMITTER: Wei Z 

PROVIDER: S-EPMC9839650 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature