Host and pathogen response to bacteriophage engineered against Mycobacterium abscessus lung infection.

ABSTRACT: Two mycobacteriophages were administered intravenously to a male with treatment-refractory Mycobacterium abscessus pulmonary infection and severe cystic fibrosis lung disease. The phages were engineered to enhance their capacity to lyse M. abscessus and were selected specifically as the most effective against the subject's bacterial isolate. In the setting of compassionate use, the evidence of phage-induced lysis was observed using molecular and metabolic assays combined with clinical assessments. M. abscessus isolates pre and post-phage treatment demonstrated genetic stability, with a general decline in diversity and no increased resistance to phage or antibiotics. The anti-phage neutralizing antibody titers to one phage increased with time but did not prevent clinical improvement through

SUBMITTER: Nick JA 

PROVIDER: S-EPMC9840467 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature