Insights into the ribosome function from the structures of non-arrested ribosome-nascent chain complexes.

ABSTRACT: During protein synthesis, the growing polypeptide threads through the ribosomal exit tunnel and modulates ribosomal activity by itself or by sensing various small molecules, such as metabolites or antibiotics, appearing in the tunnel. While arrested ribosome-nascent chain complexes (RNCCs) have been extensively studied structurally, the lack of a simple procedure for the large-scale preparation of peptidyl-tRNAs, intermediates in polypeptide synthesis that carry the growing chain, means that little attention has been given to RNCCs representing functionally active states of the ribosome. Here we report the facile synthesis of stably linked peptidyl-tRNAs through a chemoenzymatic approach based on native chemical ligation and use them to determine several structures of RNCCs in the function

SUBMITTER: Syroegin EA 

PROVIDER: S-EPMC9840698 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature