Comparison of DNA methylation profiles of human embryos cultured in either uninterrupted or interrupted incubators.

ABSTRACT:

Purpose

We aimed to compare the DNA methylation profiles of human embryos cultured in uninterrupted or interrupted incubators.

Methods

This study included 9 women, ≤ 30 years old (range: 20-30 years), without a history of genetic diseases or smoking, undergoing ICSI treatment, and each woman donated one oocyte. Embryos were randomly assigned to culture in either time-lapse imaging or standard incubators after ICSI. We compared the DNA methylation profiles of human eight-cell embryos cultured in uninterrupted condition using time-lapse imaging (TLI) incubator (EmbryoScope) to those cultured in interrupted culture model using standard incubators (SI, G185 K-System). Nine single-cell whole-genome bisulfite sequencing (WGBS) datasets were analyzed, including four SI-cultured emb

SUBMITTER: Zhu L 

PROVIDER: S-EPMC9840733 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature