Transcriptional and post-transcriptional control of autophagy and adipogenesis by YBX1.

ABSTRACT: Obesity is strongly associated with metabolic diseases, which have become a global health problem. Exploring the underlying mechanism of adipogenesis is crucial for the treatment of excess white fat. Oncogene YBX1 is a multifunctional DNA- and RNA-binding protein that regulates brown adipogenesis. However, the role of YBX1 in white adipogenesis and adipose tissue expansion remains unknown. Here, we showed that YBX1 deficiency inhibited murine and porcine adipocyte differentiation. YBX1 positively regulated adipogenesis through promoting ULK1- and ULK2-mediated autophagy. Mechanistically, we identified YBX1 serves as a 5-methylcytosine (m⁵C)-binding protein directly targeting m⁵C-containing Ulk1 mRNA by using RNA immunoprecipitation. RNA decay assay further proved that

SUBMITTER: Wu R 

PROVIDER: S-EPMC9841012 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature