Profiling the intragenic toxicity determinants of toxin-antitoxin systems: revisiting hok/Sok regulation.

ABSTRACT: Type I toxin-antitoxin systems (T1TAs) are extremely potent bacterial killing systems difficult to characterize using classical approaches. To assess the killing capability of type I toxins and to identify mutations suppressing the toxin expression or activity, we previously developed the FASTBAC-Seq (Functional AnalysiS of Toxin-Antitoxin Systems in BACteria by Deep Sequencing) method in Helicobacter pylori. This method combines a life and death selection with deep sequencing. Here, we adapted and improved our method to investigate T1TAs in the model organism Escherichia coli. As a proof of concept, we revisited the regulation of the plasmidic hok/Sok T1TA system. We revealed the death-inducing phenotype of the Hok toxin when it is expressed from the chromosome in the absence of the antit

SUBMITTER: Le Rhun A 

PROVIDER: S-EPMC9841398 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature