Design of programmable post-translational switch control platform for on-demand protein secretion in mammalian cells.

ABSTRACT: The development of novel strategies to program cellular behaviors is a central goal in synthetic biology, and post-translational control mediated by engineered protein circuits is a particularly attractive approach to achieve rapid protein secretion on demand. We have developed a programmable protease-mediated post-translational switch (POSH) control platform composed of a chimeric protein unit that consists of a protein of interest fused via a transmembrane domain to a cleavable ER-retention signal, together with two cytosolic inducer-sensitive split protease components. The protease components combine in the presence of the specific inducer to generate active protease, which cleaves the ER-retention signal, releasing the transmembrane-domain-linked protein for trafficking to the trans-Go

SUBMITTER: Mansouri M 

PROVIDER: S-EPMC9841418 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature