Development of an Impedimetric Aptasensor for Detection of Progesterone in Undiluted Biological Fluids.

ABSTRACT: A cost-effective, deployable, and quantitative progesterone biosensor is desirable for regular progesterone sensing in biological and environmental samples to safeguard public health. Aptasensors have been shown to be affordable as compared to antibody-based sensors, but so far, none of the progesterone aptamers could detect it in undiluted and unprocessed biological samples. Thus, to select an aptamer suitable for biosensing in unprocessed biological samples, a modified magnetic bead-based approach with counter-selection in milk and serum was performed. G-quadruplex forming progesterone aptamers were preferentially screened through in silico, gold nanoparticle-based adsorption-desorption assay and circular dichroism spectroscopy. GQ5 aptamer showed extended stability and a high progestero

SUBMITTER: Kumar P 

PROVIDER: S-EPMC9841775 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature