Transcriptional signature in microglia isolated from an Alzheimer's disease mouse model treated with scanning ultrasound.

ABSTRACT: Transcranial scanning ultrasound combined with intravenously injected microbubbles (SUS^+MB) has been shown to transiently open the blood-brain barrier and reduce the amyloid-β (Aβ) pathology in the APP23 mouse model of Alzheimer's disease (AD). This has been accomplished through the activation of microglial cells; however, their response to the SUS treatment is incompletely understood. Here, wild-type (WT) and APP23 mice were subjected to SUS^+MB, using nonsonicated mice as sham controls. After 48 h, the APP23 mice were injected with methoxy-XO4 to label Aβ aggregates, followed by microglial isolation into XO4⁺ and XO4^- populations using flow cytometry. Both XO4⁺ and XO4^- cells were subjected to RNA sequencing and transcripto

SUBMITTER: Leinenga G 

PROVIDER: S-EPMC9842024 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature