YAP 5-methylcytosine modification increases its mRNA stability and promotes the transcription of exosome secretion-related genes in lung adenocarcinoma.

ABSTRACT: YAP is a transcriptional co-activator with critical roles in tumorigenesis. However, its upstream regulatory mechanism, especially how its mRNA stability is regulated, remains to be further studied. Here, we validated that YAP expression was higher in lung adenocarcinoma (LUAD) tissues compared to adjacent normal tissues, and found that YAP m⁵C modification occurred in its 328-331 3' UTR region under the promotion NSUN2 and ALYREF, and increased the stability of YAP mRNA. This m⁵C modification also inhibited miR-582-3p binding and m⁶A modification in the nearby region. In addition, YAP m⁵C modification enhanced the exosome secretion effect, which was caused by two YAP-dependent transcription factors, Mycn and SOX10, and then stimulating the trans

SUBMITTER: Yu W 

PROVIDER: S-EPMC9842506 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature