Knock-out of 5-lipoxygenase in overexpressing tumor cells-consequences on gene expression and cellular function.

ABSTRACT: 5-Lipoxygenase (5-LO), the central enzyme in the biosynthesis of leukotrienes, is frequently expressed in human solid malignancies even though the enzyme is not present in the corresponding healthy tissues. There is little knowledge on the consequences of this expression for the tumor cells regarding gene expression and cellular function. We established a knockout (KO) of 5-LO in different cancer cell lines (HCT-116, HT-29, U-2 OS) and studied the consequences on global gene expression using next generation sequencing. Furthermore, cell viability, proliferation, migration and multicellular tumor spheroid (MCTS) formation were studied in these cells. Our results show that 5-LO influences the gene expression and cancer cell function in a cell type-dependent manner. The enzyme affected genes

SUBMITTER: Weisser H 

PROVIDER: S-EPMC9842508 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature