Methylation-based epigenetic studies and gene integration analysis of preeclampsia.

ABSTRACT:

Background

Preeclampsia (PE) is a multi-factor and multi-mechanism disease, which may jeopardize the life safety of affected pregnant women and fetuses. Our study aimed to detect the potential molecular indicators of PE that might be helpful for its diagnosis and treatment.

Methods

Methylation assay of PE and normal pregnancies placental biopsies was analyzed using the Illumina Human Methylation-27 Assay. Differentially expressed genes (DEGs) were analyzed using R-DESeq2 software. Subsequently, the relationship between DNA methylation genes and DEGs were evaluated. Furthermore, immunohistochemical (IHC) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) validation analyses were conducted for the hub genes.

Results

These hub genes (including

SUBMITTER: Jiang L 

PROVIDER: S-EPMC9843334 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature