Method for Improved Fluorescence Corrections for Molar Mass Characterization by Multiangle Light Scattering.

ABSTRACT: Multiangle light scattering (MALS) was used to determine the absolute molar mass of fluorescent macromolecules. It is standard protocol to install bandwidth filters before MALS detectors to suppress detection of fluorescent emissions. Fluorescence can introduce tremendous error in light scattering measurements and is a formidable challenge in accurately characterizing fluorescent macromolecules and particles. However, we show that for some systems, bandwidth filters alone are insufficient for blocking fluorescence in molar mass determinations. For these systems, we have devised a correction procedure to calculate the amount of fluorescence interference in the filtered signal. By determining the intensity of fluorescent emission not blocked by the bandwidth filters, we can correct the filte

SUBMITTER: Pittman ZA 

PROVIDER: S-EPMC9843603 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature