Recapitulating folliculogenesis and oogenesis outside the body: encapsulated in vitro follicle growth†.

ABSTRACT: Folliculogenesis is a tightly coordinated process essential for generating a fertilization-competent gamete while also producing gonadal hormones that sustain endocrine function. In vitro follicle growth systems have been critical to our understanding of key events in folliculogenesis, such as gonadotropin-independent and dependent growth, steroid hormone production, and oocyte growth and maturation (cytoplasmic and meiotic). Although there are several successful follicle culture strategies, the following protocol details an encapsulated in vitro follicle growth (eIVFG) system for use with mouse ovarian follicles. Encapsulated IVFG is performed with alginate hydrogels, which are biologically inert, maintains cell-to-cell interactions between granulosa cells and the oocyte, and preserves fo

SUBMITTER: Converse A 

PROVIDER: S-EPMC9843677 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature