Doxycycline-dependent Cas9-expressing pig resources for conditional in vivo gene nullification and activation.

ABSTRACT:

Background

CRISPR-based toolkits have dramatically increased the ease of genome and epigenome editing. SpCas9 is the most widely used nuclease. However, the difficulty of delivering SpCas9 and inability to modulate its expression in vivo hinder its widespread adoption in large animals.

Results

Here, to circumvent these obstacles, a doxycycline-inducible SpCas9-expressing (DIC) pig model was generated by precise knock-in of the binary tetracycline-inducible expression elements into the Rosa26 and Hipp11 loci, respectively. With this pig model, in vivo and/or in vitro genome and epigenome editing could be easily realized. On the basis of the DIC system, a convenient Cas9-based conditional knockout strategy was devised through controlling the expression of rtTA component by tis

SUBMITTER: Jin Q 

PROVIDER: S-EPMC9843877 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature