Development and validation of a duplex real-time PCR for the rapid detection and quantitation of HTLV-1.

ABSTRACT:

Background

The HTLV-1 prevalence in China varies geographically, while HTLV-2 infection has rarely been found so far. Proviral load is one of the determining factors of pathogenesis and progression of HTLV-1 related diseases. However, neither molecular assays nor commercial kits are available for HTLV-1 diagnosis in China. The objective of the present study was to develop and validate a TaqMan qPCR assay for HTLV-1 proviral load quantification.

Results

A plasmid containing both the HTLV-1 of interest and a fragment of the RNase P (RPPH1) gene was constructed and used to establish the standard curves. The assay has a wide dynamic range (2.5 × 10⁸ copies/reaction ~ 25 copies/reaction) and sensitive to 1 copy for HTLV-1 and RPPH1. The limit of detection for Hut102 ce

SUBMITTER: Ji H 

PROVIDER: S-EPMC9843979 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature