Transcriptomic analyses of treatment-naive pediatric ulcerative colitis patients and exploration of underlying disease pathogenesis.

ABSTRACT:

Background

Ulcerative colitis (UC) is a form of chronic inflammatory bowel disease of nonspecific origin. This study used an RNA-Sequencing (RNA-Seq) approach to evaluate the transcriptomic landscape of a well-stratified treatment-naïve pediatric UC patient population by comparing them with healthy control children. The data were analyzed to evaluate the mechanisms driving UC-related intestinal inflammation and fibrosis.

Methods

Intestinal mucosal samples from five pediatric UC patients and five healthy controls were analyzed by RNA-Seq, and results were verified by qPCR. A CRISPR/Cas9 approach was used to knock out the expression of HLA-DRB5, and molecular biology techniques were used for additional mechanistic studies.

Results

In these analyses, 2290 genes were foun

SUBMITTER: Pang X 

PROVIDER: S-EPMC9843999 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature