Development of an assay system for the analysis of host RISC activity in the presence of a potyvirus RNA silencing suppressor, HC-Pro.

ABSTRACT:

Background

To investigate the mechanism of RNA silencing suppression, the genetic transformation of viral suppressors of RNA silencing (VSRs) in Arabidopsis integrates ectopic VSR expression at steady state, which overcomes the VSR variations caused by different virus infections or limitations of host range. Moreover, identifying the insertion of the transgenic VSR gene is necessary to establish a model transgenic plant for the functional study of VSR.

Methods

Developing an endogenous AGO1-based in vitro RNA-inducing silencing complex (RISC) assay prompts further investigation into VSR-mediated suppression. Three P1/HC-Pro plants from turnip mosaic virus (TuMV) (P1/HC-Pro^Tu), zucchini yellow mosaic virus (ZYMV) (P1/HC-Pro^Zy), and tobacco etch virus (TEV

SUBMITTER: Hong SF 

PROVIDER: S-EPMC9844029 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature