Unbiased Quantification of Golgi Scattering and Golgi-Centrosome Association.

ABSTRACT: The vertebrate Golgi complex is a large dynamic organelle which undergoes morphological changes and fragmentation both as a part of normal physiological dynamics and under disease conditions. The Golgi is known to have a functionally important relationship with the centrosome. The extent of the spatial association between these two organelles varies in a dynamic and regulated manner. It is essential to have a reliable unbiased approach to evaluate Golgi volume, Golgi extension/scattering in the 3D cell space, and spatial association of the Golgi with the centrosome. It is also important that each of these features is evaluated by a simple metric, one measurement per cell, so that the variability and deviations in the cell population can be easily assessed. Here, we present an approach to a

SUBMITTER: Frye KB 

PROVIDER: S-EPMC9844073 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature