Longitudinal in vivo imaging of adult Danionella cerebrum using standard confocal microscopy.

ABSTRACT: Danionella cerebrum is a new vertebrate model that offers an exciting opportunity to visualize dynamic biological processes in intact adult animals. Key advantages of this model include its small size, life-long optical transparency, genetic amenability and short generation time. Establishing a reliable method for longitudinal in vivo imaging of adult D. cerebrum while maintaining viability will allow in-depth image-based studies of various processes involved in development, disease onset and progression, wound healing, and aging in an intact live animal. Here, a method for both prolonged and longitudinal confocal live imaging of adult D. cerebrum using custom-designed and 3D-printed imaging chambers is described. Two transgenic D. cerebrum lines were created to test the imaging system, i.

SUBMITTER: Lam PY 

PROVIDER: S-EPMC9844135 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature