Novel CaLB-like Lipase Found Using ProspectBIO, a Software for Genome-Based Bioprospection.

ABSTRACT: Enzymes have been highly demanded in diverse applications such as in the food, pharmaceutical, and industrial fuel sectors. Thus, in silico bioprospecting emerges as an efficient strategy for discovering new enzyme candidates. A new program called ProspectBIO was developed for this purpose as it can find non-annotated sequences by searching for homologs of a model enzyme directly in genomes. Here we describe the ProspectBIO software methodology and the experimental validation by prospecting for novel lipases by sequence homology to Candida antarctica lipase B (CaLB) and conserved motifs. As expected, we observed that the new bioprospecting software could find more sequences (1672) than a conventional similarity-based search in a protein database (733). Additionally, the absence of p

SUBMITTER: Breda GC 

PROVIDER: S-EPMC9844320 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature