Towards a standardized multi-tissue decellularization protocol for the derivation of extracellular matrix materials.

ABSTRACT: The goal of tissue decellularization is to efficiently remove unwanted cellular components, such as DNA and cellular debris, while retaining the complex structural and molecular milieu within the extracellular matrix (ECM). Decellularization protocols to date are centered on customized tissue-specific and lab-specific protocols that involve consecutive manual steps which results in variable and protocol-specific ECM material. The differences that result from the inconsistent protocols between decellularized ECMs affect consistency across batches, limit comparisons between results obtained from different laboratories, and could limit the transferability of the material for consistent laboratory or clinical use. The present study is the first proof-of-concept towards the development of a sta

SUBMITTER: Biehl A 

PROVIDER: S-EPMC9844390 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature