Tumor protein D52 (TPD52) affects cancer cell metabolism by negatively regulating AMPK.

ABSTRACT:

Background

The AMP-activated protein kinase (AMPK) is a central regulator of energy homeostasis, with deregulation leading to cancer and other diseases. However, how this pathway is dysregulated in cancer has not been well clarified.

Methods

Using a tandem affinity purification/mass-spec technique and biochemical analyses, we identified tumor protein D52 (TPD52) as an AMPKα-interacting molecule. To explore the biological effects of TPD52 in cancers, we conducted biochemical and metabolic assays in vitro and in vivo with cancer cells and TPD52 transgenic mice. Finally, we assessed the clinical significance of TPD52 expression in breast cancer patients using bioinformatics techniques.

Results

TPD52, initially identified to be overexpressed in many human cancers, was fou

SUBMITTER: Chen Y 

PROVIDER: S-EPMC9844640 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature