Spatially selective cell treatment and collection for integrative drug testing using hydrodynamic flow focusing and shifting.

ABSTRACT: Hydrodynamic focusing capable of readily producing and controlling laminar flow facilitates drug treatment of cells in existing microfluidic culture devices. However, to expand applications of such devices to multiparameter drug testing, critical limitations in current hydrodynamic focusing microfluidics must be addressed. Here we describe hydrodynamic focusing and shifting as an advanced microfluidics tool for spatially selective drug delivery and integrative cell-based drug testing. We designed and fabricated a co-flow focusing, three-channel microfluidic device with a wide cell culture chamber. By controlling inlet flow rates of sample and two side solutions, we could generate hydrodynamic focusing and shifting that mediated precise regulation of the path and width of reagent and drug s

SUBMITTER: Wang X 

PROVIDER: S-EPMC9844832 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature