Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells.

ABSTRACT: The generation of organoids is one of the biggest scientific advances in regenerative medicine. Here, by lengthening the time that human pluripotent stem cells (hPSCs) were exposed to a three-dimensional microenvironment, and by applying defined renal inductive signals, we generated kidney organoids that transcriptomically matched second-trimester human fetal kidneys. We validated these results using ex vivo and in vitro assays that model renal development. Furthermore, we developed a transplantation method that utilizes the chick chorioallantoic membrane. This approach created a soft in vivo microenvironment that promoted the growth and differentiation of implanted kidney organoids, as well as providing a vascular component. The stiffness of the in ovo chorioallantoic membrane microenviro

SUBMITTER: Garreta E 

PROVIDER: S-EPMC9845070 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature