Low input capture Hi-C (liCHi-C) identifies promoter-enhancer interactions at high-resolution.

ABSTRACT: Long-range interactions between regulatory elements and promoters are key in gene transcriptional control; however, their study requires large amounts of starting material, which is not compatible with clinical scenarios nor the study of rare cell populations. Here we introduce low input capture Hi-C (liCHi-C) as a cost-effective, flexible method to map and robustly compare promoter interactomes at high resolution. As proof of its broad applicability, we implement liCHi-C to study normal and malignant human hematopoietic hierarchy in clinical samples. We demonstrate that the dynamic promoter architecture identifies developmental trajectories and orchestrates transcriptional transitions during cell-state commitment. Moreover, liCHi-C enables the identification of disease-relevant cell types

SUBMITTER: Tomas-Daza L 

PROVIDER: S-EPMC9845235 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature