A genome-wide relay of signalling-responsive enhancers drives hematopoietic specification.

ABSTRACT: Developmental control of gene expression critically depends on distal cis-regulatory elements including enhancers which interact with promoters to activate gene expression. To date no global experiments have been conducted that identify their cell type and cell stage-specific activity within one developmental pathway and in a chromatin context. Here, we describe a high-throughput method that identifies thousands of differentially active cis-elements able to stimulate a minimal promoter at five stages of hematopoietic progenitor development from embryonic stem (ES) cells, which can be adapted to any ES cell derived cell type. We show that blood cell-specific gene expression is controlled by the concerted action of thousands of differentiation stage-specific sets of cis-elements which respon

SUBMITTER: Edginton-White B 

PROVIDER: S-EPMC9845378 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature