Increased plasma genistein after bariatric surgery could promote remission of NAFLD in patients with obesity.

ABSTRACT:

Background

Bariatric surgery is associated with a positive effect on the progress of non-alcoholic associated fatty liver disease (NAFLD). Although weight loss is the obvious mechanism, there are also weight-independent mechanisms.

Methods

We collected blood samples from 5 patients with obesity before and 3 months after surgery and performed an LC-MS-based untargeted metabolomics test to detect potential systemic changes. We also constructed sleeve gastrectomy (SG) mice models. The plasma, liver and intestine samples were collected and analyzed by qPCR, ELISA and HPLC. Cohousing experiments and feces transplantation experiments were performed on mice to study the effect of gut microbiota. Genistein administration experiments were used to study the in vivo function of the met

SUBMITTER: Wang G 

PROVIDER: S-EPMC9846086 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature