Intestinal epithelial cell-derived components regulate transcriptome of Lactobacillus rhamnosus GG.

ABSTRACT:

Introduction

Intestinal epithelial cells (IECs) provide the frontline responses to the gut microbiota for maintaining intestinal homeostasis. Our previous work revealed that IEC-derived components promote the beneficial effects of a commensal and probiotic bacterium, Lactobacillus rhamnosus GG (LGG). This study aimed to elucidate the regulatory effects of IEC-derived components on LGG at the molecular level.

Methods

Differential gene expression in LGG cultured with IEC-derived components at the timepoint between the exponential and stationary phase was studied by RNA sequencing and functional analysis.

Results

The transcriptomic profile of LGG cultured with IEC-derived components was significantly different from that of control LGG, with 231 genes were signific

SUBMITTER: Schalich K 

PROVIDER: S-EPMC9846326 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature