Periodic inhibition of Erk activity drives sequential somite segmentation.

ABSTRACT: Sequential segmentation creates modular body plans of diverse metazoan embryos^1-4. Somitogenesis establishes the segmental pattern of the vertebrate body axis. A molecular segmentation clock in the presomitic mesoderm sets the pace of somite formation⁴. However, how cells are primed to form a segment boundary at a specific location remains unclear. Here we developed precise reporters for the clock and double-phosphorylated Erk (ppErk) gradient in zebrafish. We show that the Her1-Her7 oscillator drives segmental commitment by periodically lowering ppErk, therefore projecting its oscillation onto the ppErk gradient. Pulsatile inhibition of the ppErk gradient can fully substitute for the role of the clock, and kinematic clock waves are dispensable for sequential segmenta

SUBMITTER: Simsek MF 

PROVIDER: S-EPMC9846577 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature