A hybrid approach to study large conformational transitions of biomolecules from single particle XFEL diffraction data.

ABSTRACT: X-ray free-electron laser (XFEL) is the latest generation of the X-ray source that could become an invaluable technique in structural biology. XFEL has ultrashort pulse duration, extreme peak brilliance, and high spatial coherence, which could enable the observation of the biological molecules in near nature state at room temperature without crystallization. However, for biological systems, due to their low diffraction power and complexity of sample delivery, experiments and data analysis are not straightforward, making it extremely challenging to reconstruct three-dimensional (3D) structures from single particle XFEL data. Given the current limitations to the amount and resolution of the data from such XFEL experiments, we propose a new hybrid approach for characterizing biomolecular conf

SUBMITTER: Asi H 

PROVIDER: S-EPMC9846856 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature