Increasing lysine level improved methanol assimilation toward butyric acid production in Butyribacterium methylotrophicum.

ABSTRACT:

Background

Methanol, a promising non-food fermentation substrate, has gained increasing interest as an alternative feedstock to sugars for the bio-based production of value-added chemicals. Butyribacterium methylotrophicum, one of methylotrophic-acetogenic bacterium, is a promising host to assimilate methanol coupled with CO₂ fixation for the production of organic acids, such as butyric acid. Although the methanol utilization pathway has been identified in B. methylotrophicum, little knowledge was currently known about its regulatory targets, limiting the rational engineering to improve methanol utilization.

Results

In this study, we found that methanol assimilation of B. methylotrophicum could be significantly improved when using corn steep liquor (CSL) as the co

SUBMITTER: Wang J 

PROVIDER: S-EPMC9847067 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature