Post-warming culture of human vitrified blastocysts with prolactin improves trophoblast outgrowth.

ABSTRACT:

Background

Human embryos express the prolactin (PRL) receptor at the morula and blastocyst stages. Treatment with PRL from cleavage to the blastocyst stage improves blastocyst outgrowth on fibronectin-coated dishes. However, whether post-warming PRL treatment of blastocysts cultured without PRL could improve outgrowth competence remains unknown. Furthermore, the optimal time for post-warming PRL treatment remains to be ascertained. This study investigated the effects of PRL treatment during recovery culture on human blastocyst outgrowth and its related genes.

Methods

In total, 374 discarded vitrified blastocysts were randomly allocated to two groups, to be cultured with (n = 208) or without PRL (control; n = 166) for 120 min for recovery, and then plated on fibronectin-coate

SUBMITTER: Ezoe K 

PROVIDER: S-EPMC9847091 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature