Position-dependent effects of RNA-binding proteins in the context of co-transcriptional splicing.

ABSTRACT: Alternative splicing is an important step in eukaryotic mRNA pre-processing which increases the complexity of gene expression programs, but is frequently altered in disease. Previous work on the regulation of alternative splicing has demonstrated that splicing is controlled by RNA-binding proteins (RBPs) and by epigenetic DNA/histone modifications which affect splicing by changing the speed of polymerase-mediated pre-mRNA transcription. The interplay of these different layers of splicing regulation is poorly understood. In this paper, we derived mathematical models describing how splicing decisions in a three-exon gene are made by combinatorial spliceosome binding to splice sites during ongoing transcription. We additionally take into account the effect of a regulatory RBP and find that th

SUBMITTER: Horn T 

PROVIDER: S-EPMC9849329 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature