A CRISPR/Cas9-mediated screen identifies determinants of early plasma cell differentiation.

ABSTRACT:

Introduction

The differentiation of B cells into antibody-secreting plasma cells depends on cell division-coupled, epigenetic and other cellular processes that are incompletely understood.

Methods

We have developed a CRISPR/Cas9-based screen that models an early stage of T cell-dependent plasma cell differentiation and measures B cell survival or proliferation versus the formation of CD138+ plasmablasts. Here, we refined and extended this screen to more than 500 candidate genes that are highly expressed in plasma cells.

Results

Among known genes whose deletion preferentially or mostly affected plasmablast formation were the transcription factors Prdm1 (BLIMP1), Irf4 and Pou2af1 (OBF-1), and the Ern1 gene encoding IRE1a, while deletion of XBP1, the transcriptional mast

SUBMITTER: Xiong E 

PROVIDER: S-EPMC9849354 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature