Hydrogen-fueled CO₂ reduction using oxygen-tolerant oxidoreductases.

ABSTRACT: Hydrogen gas obtained from cheap or sustainable sources has been investigated as an alternative to fossil fuels. By using hydrogenase (H₂ase) and formate dehydrogenase (FDH), H₂ and CO₂ gases can be converted to formate, which can be conveniently stored and transported. However, developing an enzymatic process that converts H₂ and CO₂ obtained from cheap sources into formate is challenging because even a very small amount of O₂ included in the cheap sources damages most H₂ases and FDHs. In order to overcome this limitation, we investigated a pair of oxygen-tolerant H₂ase and FDH. We achieved the cascade reaction between H₂ase from Ralstonia eutropha H16 (ReSH) and FDH from Rhodobact

SUBMITTER: Cha J 

PROVIDER: S-EPMC9849572 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature