Immunodominant antibody responses directed to SARS-CoV-2 hotspot mutation sites and risk of immune escape.

ABSTRACT:

Introduction

Considering the likely need for the development of novel effective vaccines adapted to emerging relevant CoV-2 variants, the increasing knowledge of epitope recognition profile among convalescents and afterwards vaccinated with identification of immunodominant regions may provide important information.

Methods

We used an RBD peptide microarray to identify IgG and IgA binding regions in serum of 71 COVID-19 convalescents and 18 vaccinated individuals.

Results

We found a set of immunodominant RBD antibody epitopes, each recognized by more than 30% of the tested cohort, that differ among the two different groups and are within conserved regions among betacoronavirus. Of those, only one peptide, P44 (S415-429), recognized by 68% of convalescents, presented Ig

SUBMITTER: Oliveira JR 

PROVIDER: S-EPMC9849925 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature