PD-1 blocking strategy for enhancing the anti-tumor effect of CAR T cells targeted CD105.

ABSTRACT:

Purpos

CD105 has become a promising target of immunotherapy development for highly specific expression on the neovascular surface of most types of tumor cells. In previous studies, we constructed a CAR T cell (CD105 CAR T cell) and observed significant antitumor activity. In this study, we optimized the structure of CD105 CAR to increase PD-1 antibody secretion function (CD105 × PD-1 CAR T cells).

Methods

we tested whether Increased PD-1 antibody secretion with CAR T cells targeted CD105 could promote in vitro proliferation, proinflammatory cytokine production and cytotoxicity,or not. For the in vivo experiments, we constructed a subcutaneously transplanted tumor model and placed it in NOD/SCID mice to verify the anti-tumor effect of this therapy.

Results

Our data sho

SUBMITTER: Wang X 

PROVIDER: S-EPMC9849980 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature