Construction of vascularized tissue-engineered breast with dual angiogenic and adipogenic micro-tissues.

ABSTRACT: Hydrogel-based micro-tissue engineering technique, a bottom-up approach, is promising in constructing soft tissue of large size with homogeneous spatial distribution and superior regeneration capacity compared to the top-down approach. However, most of the studies employed micro-tissues with simple mesenchymal stem cells, which could hardly meet the growth of matrix and vessels. Therefore, we recommend a dual micro-tissues assembly strategy to construct vascularized tissue-engineered breast grafts (TEBGs). Adipose micro-tissues (AMs) and vessel micro-tissues (VMs) were fabricated by seeding adipose-derived stem cells (ADSCs) and human umbilical vein endothelial cells (HUVECs) on collagen microgels (COLs) with a uniform diameter of ∼250 ​μm, respectively. TEBGs were constructed by injecting

SUBMITTER: Ni R 

PROVIDER: S-EPMC9850046 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature