Effect of the snake venom component crotamine on lymphatic endothelial cell responses and lymph transport.

ABSTRACT:

Objective

The pathology of snake envenomation is closely tied to the severity of edema in the tissue surrounding the area of the bite. Elucidating the mechanisms that promote the development of such severe edema is critical to a better understanding of how to treat this life-threatening injury. We focused on one of the most abundant venom components in North American viper venom, crotamine, and the effects it has on the cells and function of the lymphatic system.

Methods

We used RT-PCR to identify the location and relative abundance of crotamine's cellular targets (Kvα channels) within the tissues and cells of the lymphatic system. We used calcium flux, nitrate production, and cell morphometry to determine the effects of crotamine on lymphatic endothelial cells. We used trac

SUBMITTER: Si H 

PROVIDER: S-EPMC9850291 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature