Fluorescently-tagged magnetic protein nanoparticles for high-resolution optical and ultra-high field magnetic resonance dual-modal cerebral angiography.

ABSTRACT: Extremely small paramagnetic iron oxide nanoparticles (FeMNPs) (<5 nm) can enhance positive magnetic resonance imaging (MRI) contrast by shortening the longitudinal relaxation time of water (T₁), but these nanoparticles experience rapid renal clearance. Here, magnetic protein nanoparticles (MPNPs) are synthesized from protein-conjugated citric acid coated FeMNPs (c-FeMNPs) without loss of the T₁ MRI properties and tagged with fluorescent dye (f-MPNPs) for optical cerebrovascular imaging. The c-FeMNPs shows average size 3.8 ± 0.7 nm with T₁ relaxivity (r₁) of 1.86 mM^-1 s^-1 and transverse/longitudinal relaxivity ratio (r₂/r₁) of 2.53 at 11.7 T. The f-MPNPs show a

SUBMITTER: Mishra SK 

PROVIDER: S-EPMC9850399 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature