Extracellular Vesicles and Particles Modulate Proton Secretion in a Model of Human Parietal Cells.

ABSTRACT: The secretion of extracellular vesicles and particles (EVPs) is an important mechanism of cellular communication. In this work, we demonstrate a functional role of EVPs in mechanisms regulating gastric acid secretion. HGT-1 cells were used as a model system to assess proton secretion. First, in order to prove EVP secretion by HGT-1 cells, EVPs were isolated by size exclusion chromatography and characterized by nanoparticle tracking analysis, Western blot, and cryo transmission electron microscopy. For examination of the potential role of EVPs in proton secretion, HGT-1 cells were treated with pharmacological EV-inhibitors, resulting in a reduction of histamine-induced proton secretion. To demonstrate the functional role of EVPs in the mechanism of proton secretion, EVP-conditioned supernat

SUBMITTER: Mistlberger-Reiner A 

PROVIDER: S-EPMC9850724 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature