Loss of TET2 impairs endothelial angiogenesis via downregulating STAT3 target genes.

ABSTRACT:

Background

Ischemic diseases represent a major global health care burden. Angiogenesis is critical in recovery of blood flow and repair of injured tissue in ischemic diseases. Ten-eleven translocation protein 2 (TET2), a member of DNA demethylases, is involved in many pathological processes. However, the role of TET2 in angiogenesis is still unrevealed.

Methods

TET2 was screened out from three DNA demethylases involved in 5-hydroxylmethylcytosine (5-hmC) regulation, including TET1, TET2 and TET3. Knockdown by small interfering RNAs and overexpression by adenovirus were used to evaluate the role of TET2 on the function of endothelial cells. The blood flow recovery and density of capillary were analyzed in the endothelial cells-specific TET2-deficient mice. RNA sequencing was

SUBMITTER: Shi Y 

PROVIDER: S-EPMC9850815 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature