Evaluation of a biotin-based surrogate virus neutralization test for detecting postvaccination antibodies against SARS-CoV-2 variants in sera.

ABSTRACT: A severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) surrogate virus neutralization test (sVNT) was used to determine the degree of inhibition of binding between human angiotensin converting enzyme 2 (hACE2) and the receptor binding domain (RBD) of spike protein by neutralizing antibodies in a biosafety level 2 facility. Here, to improve the sensitivity and specificity of the commercial sVNT, we developed a new biotin based sVNT using biotinylated RBD and HRP conjugated streptavidin instead of HRP conjugated RBD for direct detection in an ELISA assay that strongly correlated to the FDA approved cPass sVNT commercial kit (R² = 0.8521) and pseudo virus neutralization test (R² = 0.9006) (pVNT). The biotin based sVNT was evaluated in 535 postvaccination serum sam

SUBMITTER: Ahn MJ 

PROVIDER: S-EPMC9850842 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature