Simplified Purification of Glycoprotein-Modified Ferritin Nanoparticles for Vaccine Development.

ABSTRACT: Ferritin-based, self-assembling protein nanoparticle vaccines are being developed against a range of viral pathogens, including SARS-CoV-2, influenza, HIV-1, and Epstein-Barr virus. However, purification of these nanoparticles is often laborious and requires customization for each potential nanoparticle vaccine. We propose that the simple insertion of a polyhistidine tag into exposed flexible loops on the ferritin surface (His-Fer) can mitigate the need for complex purifications and enable facile metal-chelate-based purification, thereby allowing for optimization of early stage vaccine candidates. Using sequence homology and computational modeling, we identify four sites that can accommodate insertion of a polyhistidine tag and demonstrate purification of both hemagglutinin-modified and SA

SUBMITTER: Weidenbacher P 

PROVIDER: S-EPMC9850919 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature