Tritium Labeling of Neuromedin S by Conjugation with [³H]N-Succinimidyl Propionate.

ABSTRACT: The human neuropeptide neuromedin S (NMS) consists of 33 amino acids. The introduction of tritium atoms into NMS has not been described so far. This represents a gap for using [³H]NMS in radioreceptor binding assays or in tracking and monitoring their metabolic pathway. Two approaches for the incorporation of tritium into NMS were explored in this study: (1) halogenation at the His-18 residue followed by catalyzed iodine-127/tritium exchange and (2) conjugation of tritiated N-succinimidyl-[2,3-³H₃]propionate ([³H]NSP) to at least one of the three available primary amines of amino acids Ile-1, Lys-15, and Lys-16 in the peptide sequence. Although iodination of histidine was achieved, subsequent iodine-127/deuterium exchange was unsuccessful.

SUBMITTER: Edelmann MR 

PROVIDER: S-EPMC9851027 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature